Spatial Interrelationships Between Proteoglycans and Extracellular Matrix Proteins in Cell Cultures

Exp Cell Res. 1985 Jun;158(2):321-32. doi: 10.1016/0014-4827(85)90457-4.

Abstract

Immunolabelling of cultured cells for chondroitin-sulfate proteoglycan (CSPG), in conjunction with antibodies to fibronectin, collagen and laminin, revealed the spatial interrelationships between the different matrix components. CSPG was organized in two major forms. Fibronectin-independent dotted patterns of CSPG were detected on the substrate and cell surfaces at early stages after plating. At later stages, however, significant overlapping was found between the two extracellular matrix components. Immunoelectron microscopic examination indicated that the CSPG was organized as granules of varying sizes which were associated with the cell surface, the substrate, or with the periphery of the fibronectin network.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Chick Embryo
  • Chondroitin Sulfate Proteoglycans / metabolism
  • Collagen / metabolism
  • Extracellular Matrix / metabolism
  • Extracellular Matrix / ultrastructure*
  • Fibronectins / metabolism
  • Fluorescent Antibody Technique
  • Laminin / metabolism
  • Microscopy, Electron
  • Proteins / metabolism*
  • Proteoglycans / metabolism*
  • Tissue Distribution

Substances

  • Chondroitin Sulfate Proteoglycans
  • Fibronectins
  • Laminin
  • Proteins
  • Proteoglycans
  • Collagen