Biotin-avidin amplified ELISA for quantitation of human IgA

J Immunoassay. 1985;6(1-2):67-77. doi: 10.1080/01971528508063021.


A biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) for the measurement of human IgA in serum, colostrum, gastric juice, and supernatants of in vitro mitogen-stimulated human peripheral blood mononuclear cells is described. The ELISA reproducibly detects as little as one nanogram of IgA in a fifty microliter sample (20 ng/ml).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Avidin
  • Biotin
  • Colostrum / immunology
  • Enzyme-Linked Immunosorbent Assay*
  • Gastric Juice / immunology
  • Humans
  • Immunoenzyme Techniques*
  • Immunoglobulin A / analysis*
  • Leukocytes / immunology
  • Milk, Human / immunology


  • Immunoglobulin A
  • Avidin
  • Biotin