Abstract
A biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) for the measurement of human IgA in serum, colostrum, gastric juice, and supernatants of in vitro mitogen-stimulated human peripheral blood mononuclear cells is described. The ELISA reproducibly detects as little as one nanogram of IgA in a fifty microliter sample (20 ng/ml).
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Avidin
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Biotin
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Colostrum / immunology
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Enzyme-Linked Immunosorbent Assay*
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Gastric Juice / immunology
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Humans
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Immunoenzyme Techniques*
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Immunoglobulin A / analysis*
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Leukocytes / immunology
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Milk, Human / immunology
Substances
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Immunoglobulin A
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Avidin
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Biotin