Peptide-Carrier Conjugation

Methods Mol Biol. 2024:2821:57-63. doi: 10.1007/978-1-0716-3914-6_4.

Abstract

To produce antibodies against synthetic peptides, it is necessary to couple them to a protein carrier. This chapter provides a nonspecialist overview of peptide-carrier conjugation. Furthermore, a protocol for coupling cysteine-containing peptides to bovine serum albumin is outlined.

Keywords: Bovine serum albumin; Peptide-carrier conjugation; m-maleimidobenzoyl-N-hydroxysuccinimidyl ester (MBS).

MeSH terms

  • Animals
  • Cattle
  • Cysteine* / chemistry
  • Peptides* / chemistry
  • Serum Albumin, Bovine* / chemistry

Substances

  • Peptides
  • Serum Albumin, Bovine
  • Cysteine