Nucleotide sequence of the gene ereA encoding the erythromycin esterase in Escherichia coli

Gene. 1985;35(3):271-8. doi: 10.1016/0378-1119(85)90005-8.


We have cloned and determined the nucleotide sequence of the gene ereA of plasmid pIP1100 which confers high-level resistance to erythromycin (Em) in Escherichia coli. The gene was defined by initiation and termination codons and by in vitro insertion-inactivation into an open reading frame (ORF) of 1032 bp corresponding to a product with an Mr of 37 765. However, the enzyme, an Em esterase, displayed an apparent Mr of 43 000 upon electrophoresis of a minicell extract on the SDS-polyacrylamide gels. The G + C content (50.5%) of the gene ereA and the preferential codon usage in its ORF suggest that this resistance determinant should be indigenous to E. coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carboxylic Ester Hydrolases / genetics*
  • Cloning, Molecular
  • Drug Resistance, Microbial
  • Erythromycin*
  • Escherichia coli / genetics*
  • Esterases / genetics*
  • Genes
  • Genes, Bacterial
  • Plasmids


  • Erythromycin
  • Esterases
  • Carboxylic Ester Hydrolases
  • erythromycin esterase

Associated data

  • GENBANK/M11277