Recombinant Biogenesis and Analysis of Cytochrome c Species

Alicia N Kreiman; Tania Yeasmin; Molly C Sutherland

doi:10.1007/978-1-0716-4043-2_10

Recombinant Biogenesis and Analysis of Cytochrome c Species

Methods Mol Biol. 2024:2839:195-211. doi: 10.1007/978-1-0716-4043-2_10.

Authors

Alicia N Kreiman¹, Tania Yeasmin², Molly C Sutherland³

Affiliations

¹ Department of Biological Sciences, University of Delaware, Newark, DE, USA.
² Department of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.
³ Department of Biological Sciences, University of Delaware, Newark, DE, USA. msuther@udel.edu.

PMID: 39008254
DOI: 10.1007/978-1-0716-4043-2_10

Abstract

Recombinant expression and biogenesis of cytochrome c species is a simple and efficient method for the production of holocytochrome c species, thus presenting an avenue for the study of cytochrome c or the cytochrome c biogenesis pathways responsible for heme attachment. Here, we describe a method for recombinant E. coli production of holocytochrome c utilizing the System I (CcmABCDEFGH) bacterial cytochrome c biogenesis pathway, followed by analysis of cytochrome c species by cell lysis and heme stain.

Keywords: Cytochrome c; Cytochrome c biogenesis; Heme; Heme stain.

MeSH terms

Cytochromes c* / biosynthesis
Cytochromes c* / genetics
Cytochromes c* / metabolism
Escherichia coli* / genetics
Escherichia coli* / metabolism
Heme* / biosynthesis
Heme* / metabolism
Recombinant Proteins* / genetics
Recombinant Proteins* / metabolism

Substances

Cytochromes c
Recombinant Proteins
Heme

Abstract

MeSH terms

Substances

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