LILRB4 regulates multiple myeloma development through STAT3-PFKFB1 pathway

Cell Death Dis. 2024 Jul 18;15(7):515. doi: 10.1038/s41419-024-06883-4.

Abstract

Although multiple myeloma (MM) responds well to immunotherapeutic treatment, certain portions of MM are still unresponsive or relapse after immunotherapy. Other immune molecules are needed for the immunotherapy of MM. Here, we revealed that leukocyte immunoglobulin-like receptor B4 (LILRB4) was highly expressed in multiple myeloma cell lines and patient samples and that the expression of LILRB4 was adversely correlated with the overall survival of MM patients. Knockdown of LILRB4 efficiently delayed the growth of MM cells both in vitro and in vivo. Mechanistically, IKZF1 transactivated LILRB4 expression to trigger the downstream of STAT3-PFKFB1 pathways to support MM cell proliferation. Blockade of LILRB4 signaling by blocking antibodies can effectively inhibit MM progression. Our data show that targeting LILRB4 is potentially an additional therapeutic strategy for the immunotherapeutic treatment of MM.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Proliferation
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Ikaros Transcription Factor / genetics
  • Ikaros Transcription Factor / metabolism
  • Male
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Mice
  • Multiple Myeloma* / genetics
  • Multiple Myeloma* / metabolism
  • Multiple Myeloma* / pathology
  • Receptors, Immunologic* / genetics
  • Receptors, Immunologic* / metabolism
  • STAT3 Transcription Factor* / metabolism
  • Signal Transduction*

Substances

  • STAT3 Transcription Factor
  • Receptors, Immunologic
  • LILRB4 protein, human
  • STAT3 protein, human
  • Ikaros Transcription Factor
  • Membrane Glycoproteins
  • IKZF1 protein, human