Doughnut-shaped bimetallic Cu-Zn-MOF with peroxidase-like activity for colorimetric detection of glucose and antibacterial applications

Talanta. 2024 Nov 1:279:126544. doi: 10.1016/j.talanta.2024.126544. Epub 2024 Jul 14.

Abstract

Metal-organic frameworks (MOFs), especially bimetallic MOFs, have attracted widespread attention for simulating the structure and function of natural enzymes. In this study, different morphologies of bimetallic Cu-Zn-MOF with different peroxidase (POD)-like activities were prepared by simply controlling the molar ratio of Cu2+ and Zn2+. Among them, the doughnut-shaped Cu9-Zn1-MOF exhibited the largest POD-like activity. Cu9-Zn1-MOF was combined with glucose oxidase to construct a sensitive and selective glucose colorimetric biosensor with a linear detection range of 10-300 μM and a detection limit of 7.1 μm. Furthermore, Cu9-Zn1-MOF can efficiently convert hydrogen peroxide (H2O2) into hydroxyl radicals that effectively kill both gram-negative and gram-positive bacteria at low H2O2 level. The results of this study may promote the synthesis of bimetallic MOFs and broaden their applications in the biomedical field.

Keywords: Antibacterial; Bimetallic; Cu–Zn-MOF; Glucose; Peroxidase-like.

MeSH terms

  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Biosensing Techniques / methods
  • Colorimetry* / methods
  • Copper* / chemistry
  • Glucose Oxidase* / chemistry
  • Glucose Oxidase* / metabolism
  • Glucose* / analysis
  • Glucose* / chemistry
  • Hydrogen Peroxide* / chemistry
  • Limit of Detection
  • Metal-Organic Frameworks* / chemistry
  • Microbial Sensitivity Tests
  • Peroxidase / chemistry
  • Peroxidase / metabolism
  • Zinc* / chemistry

Substances

  • Copper
  • Metal-Organic Frameworks
  • Zinc
  • Anti-Bacterial Agents
  • Glucose
  • Glucose Oxidase
  • Hydrogen Peroxide
  • Peroxidase