Abstract
The enhancing effect of vitamin C on the conversion of arachidonic acid, endoperoxide G2 and endoperoxide H2 to 6-keto-PGF 1 alpha, the stable metabolite of prostacyclin, by ram seminal vesicle microsomes was further investigated. From the incubations of these substrates with 1-tryptophan, catalase, superoxide dismutase and 15-HPETE it became clear that vitamin C apparently acts mainly through neutralization of the oxidative species formed during the reduction of endoperoxide G2 to endoperoxide H2. Although it has also a more direct stimulating activity on the prostacyclin synthase, a possible interference with hydroperoxy derivatives of arachidonic acid cannot be completely ruled out.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arachidonic Acids / pharmacology
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Ascorbic Acid / pharmacology*
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Catalase / pharmacology
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Epoprostenol / biosynthesis*
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Epoprostenol / pharmacology
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In Vitro Techniques
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Leukotrienes*
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Lipid Peroxides / pharmacology
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Male
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Microsomes / metabolism*
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Prostaglandin Endoperoxides, Synthetic / metabolism
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Prostaglandin H2
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Prostaglandins G / metabolism
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Prostaglandins H / metabolism
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Seminal Vesicles / metabolism*
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Sheep
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Superoxide Dismutase / pharmacology
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Tryptophan / pharmacology
Substances
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Arachidonic Acids
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Leukotrienes
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Lipid Peroxides
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Prostaglandin Endoperoxides, Synthetic
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Prostaglandins G
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Prostaglandins H
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Prostaglandin H2
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prostaglandin G2
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15-hydroperoxy-5,8,11,13-eicosatetraenoic acid
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Tryptophan
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Epoprostenol
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Catalase
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Superoxide Dismutase
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Ascorbic Acid