Efficient Knock-in by CRISPR/Cas9-mediated homology-directed repair in Caenorhabditis briggsae

Abstract

The CRISPR/Cas9 technology opens new avenues for detailed genetic exploration and comparative genomics. However, the current application of CRISPR/Cas9 in C. briggsae especially for homology-directed repair (HDR) knock-in (KI) still encounter significant challenges. In this study, we demonstrate that employing high concentration HDR donor vectors significantly improves KI efficiency in C. briggsae .