RNA Sequencing Experimental Analysis Workflow Using Caenorhabditis elegans

Methods Mol Biol. 2024:2812:115-141. doi: 10.1007/978-1-0716-3886-6_6.

Abstract

RNA sequencing is an approach to transcriptomic profiling that enables the detection of differentially expressed genes in response to genetic mutation or experimental treatment, among other uses. Here we describe a method for the use of a customizable, user-friendly bioinformatic pipeline to identify differentially expressed genes in RNA sequencing data obtained from C. elegans, with attention to the improvement in reproducibility and accuracy of results.

Keywords: Bioinformatic pipeline; Differentially expressed genes; RNA sequencing; Reproducibility.

MeSH terms

  • Animals
  • Caenorhabditis elegans* / genetics
  • Computational Biology* / methods
  • Gene Expression Profiling* / methods
  • High-Throughput Nucleotide Sequencing / methods
  • Reproducibility of Results
  • Sequence Analysis, RNA* / methods
  • Software*
  • Transcriptome
  • Workflow*