RNA sequencing is an approach to transcriptomic profiling that enables the detection of differentially expressed genes in response to genetic mutation or experimental treatment, among other uses. Here we describe a method for the use of a customizable, user-friendly bioinformatic pipeline to identify differentially expressed genes in RNA sequencing data obtained from C. elegans, with attention to the improvement in reproducibility and accuracy of results.
Keywords: Bioinformatic pipeline; Differentially expressed genes; RNA sequencing; Reproducibility.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.