The alpha cell response to glucose change during perfusion of anti-insulin serum in pancreas isolated from normal rats

Diabetologia. 1985 Nov;28(11):836-40. doi: 10.1007/BF00291074.

Abstract

To determine the effect of neutralization of endogenous insulin upon the glucagon response to a rise and fall of glucose concentration, pancreata isolated from normal rats were perfused with either a potent anti-pork insulin guinea pig serum or a nonimmune guinea pig serum for 30 min. During this period glucose concentration was changed from 100 mg/dl to either 130, 180 or 80 mg/dl for 10 min. Antiserum perfusion at 100 mg/dl caused an approximately two-fold increase in glucagon which was not suppressed by an increase in glucose concentration to either 130 or 180 mg/dl, although glucagon secretion was significantly suppressed in the control experiments in which nonimmune serum was perfused. However, the 0.38 +/- 0.21 ng/min rise in glucagon secretion in response to a reduction in glucose concentration to 80 mg/dl in the control experiments was not abolished by antiserum perfusion but, instead, was enhanced (2.66 +/- 0.60 ng/min). These findings suggest that insulin may be required for glucose-mediated suppression of glucagon in the isolated pancreas of normal rats but not for stimulation of glucagon secretion by mild glucopenia. Alternatively, neutralization of insulin-mediated release-inhibition of glucagon secretion may simply have altered alpha cell responsiveness in a direction that desensitized it nonspecifically to suppression and sensitized it to stimulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Glucagon / metabolism
  • Glucose / administration & dosage
  • Glucose / pharmacology*
  • In Vitro Techniques
  • Insulin / physiology
  • Insulin Antibodies / physiology*
  • Islets of Langerhans / drug effects*
  • Male
  • Rats
  • Rats, Inbred Strains

Substances

  • Insulin
  • Insulin Antibodies
  • Glucagon
  • Glucose