Effect of green tea extract antioxidant on dentin shear bond strength and resin-tag penetration depth after non-vital bleaching

F1000Res. 2023 Sep 8:12:660. doi: 10.12688/f1000research.133313.2. eCollection 2023.

Abstract

Background: 35% Hydrogen peroxide (H 2O 2) as an active material for internal bleaching can produce free radicals that can affect resin tag penetration into the dentinal tubules. Application of 10% and 35% green tea (GT) extract as an antioxidant after 2 minutes are expected to remove free radical residues and increase dentin shear bond strength and resin tags penetration depth after non-vital bleaching. Methods: 30 extracted healthy human premolars were cut horizontally 2 mm from the Cemento Enamel Junction margin to the crown part, then cut in a mesio-distal direction into two parts. The specimens were divided into five groups: normal dentin, post bleaching dentin, delayed 2 weeks, 10% GT, and 35% GT group. Non-vital walking bleach with 35% H 2O 2 gel was done to all groups except control group. Soon after, 10% and 35% GT extract gel were applied on dentin for 2 minutes, then the specimens were rinsed-off with aquabidest for 2 minutes and dried. All specimens were etched and bonded with an etch-and-rinse adhesive system and filled with resin composite. The shear bond strength assessment was carried out using a Universal Testing Machine (UTM) with a cross-head speed of 0.5 mm/minute. Confocal laser scanning microscopy (CLSM) with a wavelength of 560 nm and a lens magnification of 40x was used to analyze the resin tag penetration. Data were analyzed by one way ANOVA and t-test. Results: There was a significant difference in resin tag penetration depth and shear bond strength between applying 10% and 35% GT extract (p < 0.05). The 35% GT extract group resulted in a significantly longer resin tag penetration than the 10% GT extract group. Conclusions: The application of 35% GT extract is more effective than 10% GT extract as an antioxidant for increasing the shear bond strength of composite resin after internal bleaching.

Keywords: Tooth bleaching; antioxidant; green tea extract.

MeSH terms

  • Antioxidants* / chemistry
  • Antioxidants* / pharmacology
  • Dental Bonding / methods
  • Dentin* / chemistry
  • Dentin* / drug effects
  • Humans
  • Plant Extracts* / chemistry
  • Resins, Synthetic / chemistry
  • Shear Strength*
  • Tea* / chemistry
  • Tooth Bleaching* / methods

Substances

  • Antioxidants
  • Tea
  • Plant Extracts
  • Resins, Synthetic

Associated data

  • figshare/10.6084/m9.figshare.22568338.v1

Grants and funding

This study was financially supported by a grant from University of Indonesia PUTI Q3 with contract number NKB-248/UN2.RST/HKP.05.00/2022.