DNA sequence of the gene for the outer membrane lipoprotein of E. coli: an extremely AT-rich promoter

Cell. 1979 Dec;18(4):1109-17. doi: 10.1016/0092-8674(79)90224-1.


The outer membrane lipoprotein is the most abundant protein in an E. coli cell. Its structural gene (Ipp) was cloned into a lambda phage vector and the nucleotide sequence of a DNA fragment of 814 bp encompassing the Ipp gene was determined. The promoter region of the gene was found to have the following features. First, a segment of 261 bp preceding the transcription initiation site (-1 to -261) has a very high AT content of 70%, in contrast to 53% for the mRNA region of 322 bp, 44% for a segment of 127 bp after the transcription termination site and 49% for the average AT content of the E. coli chromosome. Second, in particular, of the first 45 bp upstream from the transcription initiation site (-1 to -45), 36 bases (80%) are A or T. Third, there is a heptanucleotide sequence homologous to the "Pribnow box," eight bases apart from the transcription initiation site. Fourth, a sequence homologous to the "RNA polymerase recognition site" exists on both strands between positions -27 and -39. Finally, there is a long dyad symmetry centered at the transcription initiation site.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenine / analysis
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics*
  • Lipoproteins / genetics*
  • Membrane Proteins / genetics*
  • Operon*
  • Thymine / analysis
  • Transcription, Genetic


  • Bacterial Proteins
  • DNA, Bacterial
  • Lipoproteins
  • Membrane Proteins
  • Adenine
  • Thymine

Associated data

  • GENBANK/J01645