Using Multiphoton Intravital Microscopy to Study Neutrophil Transmigration and Blood-Brain Barrier Permeability in a Mouse Model of Herpes Simplex Virus Encephalitis

Laura Bricio-Moreno; Evelyn A Kurt-Jones; Elizabeth W Sorensen; Andrew D Luster; Benedict D Michael

doi:10.1007/978-1-0716-4023-4_5

Using Multiphoton Intravital Microscopy to Study Neutrophil Transmigration and Blood-Brain Barrier Permeability in a Mouse Model of Herpes Simplex Virus Encephalitis

Methods Mol Biol. 2024:2828:45-55. doi: 10.1007/978-1-0716-4023-4_5.

Authors

Laura Bricio-Moreno¹, Evelyn A Kurt-Jones², Elizabeth W Sorensen¹, Andrew D Luster¹, Benedict D Michael^{3

4

5}

Affiliations

¹ Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA.
² Department of Medicine, Division of Infectious Disease and Immunology, University of Massachusetts Chan Medical School, Worcester, MA, USA.
³ Department of Neurology, The Walton Centre NHS Foundation Trust, Liverpool, UK. Benedict.Michael@liverpool.ac.uk.
⁴ The National Institute for Health Research Health Protection Research Unit for Emerging and Zoonotic Infections, University of Liverpool, Liverpool, UK. Benedict.Michael@liverpool.ac.uk.
⁵ Department of Clinical Infection Microbiology and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, University of Liverpool, Liverpool, UK. Benedict.Michael@liverpool.ac.uk.

PMID: 39147969
DOI: 10.1007/978-1-0716-4023-4_5

Abstract

Multiphoton intravital microscopy (MP-IVM) is an imaging technique used for the observation of living organisms at a microscopic resolution. The tissue of interest is exposed through a window allowing imaging of cells in real time. Using MP-IVM, the temporospatial kinetics of leukocyte transendothelial migration can be visualized and quantitated using reporter mice and cell-specific fluorophore-conjugated monoclonal antibodies to track the leukocytes within and outside of vascular beds. Here we describe a method used to study neutrophil transendothelial migration and blood-brain barrier permeability in a mouse model of herpes simplex virus I (HSV) encephalitis.

Keywords: Blood−brain barrier permeability; Brain parenchyma; HSV encephalitis; Leukocyte migration; Multiphoton intravital microscopy; Neutrophil migration; Transendothelial migration.

MeSH terms

Animals
Blood-Brain Barrier* / metabolism
Blood-Brain Barrier* / pathology
Blood-Brain Barrier* / virology
Disease Models, Animal*
Encephalitis, Herpes Simplex* / metabolism
Encephalitis, Herpes Simplex* / pathology
Encephalitis, Herpes Simplex* / virology
Herpesvirus 1, Human / physiology
Intravital Microscopy* / methods
Mice
Microscopy, Fluorescence, Multiphoton* / methods
Neutrophils* / metabolism
Permeability
Transendothelial and Transepithelial Migration*