Mesorhizobium koreense sp. nov., Isolated from Soil

J Microbiol Biotechnol. 2024 Sep 28;34(9):1819-1825. doi: 10.4014/jmb.2404.04026. Epub 2024 Jun 24.

Abstract

An aerobic, Gram-stain-negative, catalase-positive, rod-shaped, and motile bacteria, designated as a strain WR6T was isolated from soil in Republic of Korea. Strain WR6T grew at temperatures of 10-37°C, at pH of 5.0-9.0, and at NaCl concentrations of 0-3.0% (w/v). Phylogenetic and 16S rRNA gene nucleotide sequence analysis confirmed that strain WR6T affiliated to the genus Mesorhizobium, with the nearest relative being Mesorhizobium waimense ICMP 19557T (98.5%). The genome of strain WR6T was 5,035,462 bp with DNA G+C content of 62.6%. In strain WR6T, Q-10 was sole ubiquinone; summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C19:0 cyclo ω8c were predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were major polar lipids. Based on these polyphasic taxonomic data, strain WR6T represents a novel species in the genus Mesorhizobium. Accordingly, we propose the name Mesorhizobium koreense sp. nov., with the type strain WR6T (=KCTC 92695T =NBRC 116021T).

Keywords: Mesorhizobium koreense sp. nov. soil; Phyllobacteriaceae; Pseudomonadota; phylogenetics.

MeSH terms

  • Bacterial Typing Techniques*
  • Base Composition*
  • DNA, Bacterial* / genetics
  • Fatty Acids* / analysis
  • Genome, Bacterial
  • Mesorhizobium* / classification
  • Mesorhizobium* / genetics
  • Mesorhizobium* / isolation & purification
  • Phospholipids / analysis
  • Phylogeny*
  • RNA, Ribosomal, 16S* / genetics
  • Republic of Korea
  • Sequence Analysis, DNA*
  • Soil Microbiology*
  • Ubiquinone* / analysis
  • Ubiquinone* / chemistry

Substances

  • RNA, Ribosomal, 16S
  • Fatty Acids
  • DNA, Bacterial
  • Ubiquinone
  • Phospholipids