B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo

Science. 1985 Jan 11;227(4683):134-40. doi: 10.1126/science.3917574.


The mouse heavy chain immunoglobulin gene contains a tissue-specific enhancer. The enhancer and flanking sequences were studied in vivo by carrying out dimethyl sulfate protection experiments on living cells, in combination with genomic sequencing. Relative to reactions on naked DNA, there are changes (protections and enhancements) in the reactivity of guanine residues to dimethyl sulfate within the enhancer sequence in myeloma, B, and early B cells, whereas virtually no alterations appear in cells of non-B lineage. Most of the affected residues are in four clusters, in sequences homologous to the octamer 5'CAGGTGGC 3' (C, cytosine; A, adenine; G. guanine; T, thymine). The alterations in the pattern of G reactivity are consistent with the tissue-specific binding of molecules to the mouse immunoglobulin heavy chain enhancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism*
  • Base Sequence
  • Cell Line
  • Enhancer Elements, Genetic*
  • Genes, Regulator*
  • Guanine / metabolism
  • Immunoglobulin Heavy Chains / genetics*
  • Methylation
  • Mice
  • Multiple Myeloma / genetics
  • RNA, Messenger / metabolism
  • Sulfuric Acid Esters / metabolism


  • Immunoglobulin Heavy Chains
  • RNA, Messenger
  • Sulfuric Acid Esters
  • Guanine
  • dimethyl sulfate

Associated data

  • GENBANK/M12827