DNA-directed in vitro synthesis and assembly of the form II D-ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides

J Bacteriol. 1985 Jan;161(1):307-13. doi: 10.1128/JB.161.1.307-313.1985.

Abstract

A biochemical analysis of the in vitro assembly of the form II ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides after transcription and translation from cloned DNA is presented. The predominant enzymatically active oligomeric forms of the in vitro-synthesized and -assembled ribulose-1,5-bisphosphate carboxylase are tetramers and hexamers. Assembly of the monomeric subunits to form active enzyme appears to be dependent on the presence of a minimum number of subunits in the cell extract. Assembly of ribulose-1,5-bisphosphate carboxylase also was observed when the protein-synthesizing extracts were prepared from cells which were partially derepressed for ribulose-1,5-bisphosphate carboxylase expression.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA, Bacterial / metabolism*
  • DNA, Recombinant / metabolism*
  • Kinetics
  • Macromolecular Substances
  • Oxygenases / biosynthesis*
  • Protein Biosynthesis
  • Rhodobacter sphaeroides / enzymology
  • Rhodobacter sphaeroides / genetics*
  • Rhodospirillum rubrum / genetics
  • Ribulose-Bisphosphate Carboxylase / biosynthesis*
  • Transcription, Genetic

Substances

  • DNA, Bacterial
  • DNA, Recombinant
  • Macromolecular Substances
  • Oxygenases
  • Ribulose-Bisphosphate Carboxylase