Yeast Two-Hybrid Assay for Investigating Antiviral Innate Immunity

Sisi Xia; Xiaoping Huo; Chunfu Zheng; Jun Chen

doi:10.1007/978-1-0716-4108-8_21

Yeast Two-Hybrid Assay for Investigating Antiviral Innate Immunity

Methods Mol Biol. 2025:2854:213-220. doi: 10.1007/978-1-0716-4108-8_21.

Authors

Sisi Xia^{1

2}, Xiaoping Huo³, Chunfu Zheng⁴, Jun Chen^{5

6}

Affiliations

¹ Department of Biological Engineering, Wuhan Polytechnic University, Wuhan, China.
² State Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, China.
³ Key Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou, China.
⁴ Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
⁵ State Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, China. chenjun@jnu.edu.cn.
⁶ Key Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou, China. chenjun@jnu.edu.cn.

PMID: 39192132
DOI: 10.1007/978-1-0716-4108-8_21

Abstract

Yeast two-hybrid (YTH) technology is a powerful tool for studying protein interactions and has been widely used in various fields of molecular biology, including the study of antiviral innate immunity. This chapter presents detailed information and experimental procedures for identifying virus-host protein interactions involved in immune regulation using yeast two-hybrid technology.

Keywords: Antiviral innate immunity; Virus-host interaction; Yeast two-hybrid (YTH).

MeSH terms

Host-Pathogen Interactions* / immunology
Humans
Immunity, Innate*
Protein Binding
Protein Interaction Mapping / methods
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / immunology
Two-Hybrid System Techniques*
Viral Proteins / immunology
Viral Proteins / metabolism

Substances

Viral Proteins