gamma-Interferon stimulates production of 1,25-dihydroxyvitamin D3 by normal human macrophages

Biochem Biophys Res Commun. 1985 Mar 15;127(2):596-603. doi: 10.1016/s0006-291x(85)80202-3.


We show for the first time that normal human pulmonary alveolar macrophages (PAM) markedly enhance their basal rate of the production of [3H]-1,25(OH)2D3 when cultured in the presence of recombinant gamma-interferon (gamma-IFN). The rate of conversion of [3H]-25(OH)D3 to [3H]-1,25(OH)2D3 was dose-dependent in a linear fashion. A maximal production of 1,25(OH)2D3 by PAM occurred after exposure of PAM to gamma-IFN for one day. This maximum plateau-level was sustained for at least five days. The authenticity of the putative 1,25(OH)2D3 obtained from PAM was tested by demonstrating the exact comigration of [3H]-1,25(OH)2D3 with chemically synthesized 1,25(OH)2D3 in four different HPLC-systems.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcitriol / biosynthesis*
  • Chromatography, High Pressure Liquid / methods
  • Humans
  • In Vitro Techniques
  • Interferon-gamma / pharmacology*
  • Lymphocytes / metabolism
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Mice
  • Mice, Inbred C3H
  • Monocytes / metabolism
  • Peritoneal Cavity / cytology
  • Pulmonary Alveoli / cytology


  • Interferon-gamma
  • Calcitriol