Differential effects of 7,8-benzoflavone on polycyclic aromatic hydrocarbon dependent mutagenesis and cytotoxicity in a keratinocyte cell-mediated mutation assay

Cancer Lett. 1985 Mar;26(2):215-20. doi: 10.1016/0304-3835(85)90029-1.

Abstract

A murine keratinocyte cell-mediated mutagenesis assay was used to examine the effects of 7,8-benzoflavone (7,8-BF) on the metabolic activation of 7,12-dimethylbenz[a]anthracene (DMBA) and benzo[a]pyrene (BP) to mutagenic and cytotoxic metabolites. DMBA-dependent mutagenesis and cytotoxicity were strongly and coordinately inhibited by 7,8-BF in a concentration-dependent manner. BP-dependent mutagenesis was also strongly inhibited by 7,8-BF in a concentration-dependent manner; whereas BP-dependent cytotoxicity was relatively unaffected by 7,8-BF. Maximum inhibition of DMBA- and BP-dependent mutagenesis occurred when the cultured keratinocytes were treated with 7,8-BF immediately prior to the addition of BP or DMBA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene / metabolism*
  • Animals
  • Benzo(a)pyrene / metabolism*
  • Benzoflavones / pharmacology*
  • Biotransformation
  • Cells, Cultured
  • Cricetinae
  • Cricetulus
  • Epidermis / metabolism*
  • Flavonoids / pharmacology*
  • Mice
  • Mutagenicity Tests
  • Mutagens / metabolism*

Substances

  • Benzoflavones
  • Flavonoids
  • Mutagens
  • Benzo(a)pyrene
  • 9,10-Dimethyl-1,2-benzanthracene
  • alpha-naphthoflavone