Isolation of the proto-oncogene c-myb from D. melanogaster

Cell. 1985 Jun;41(2):449-56. doi: 10.1016/s0092-8674(85)80018-0.

Abstract

We have isolated the proto-oncogene c-myb from Drosophila melanogaster. This gene is represented by a single locus at position 13E-F on the X chromosome, and is expressed in early embryos by transcription into two polyadenylated RNAs with lengths of approximately 3.0 and 3.8 kb. The gene may encode a protein with a molecular weight of at least 55,000 that shares a domain with c-myb (chicken) in which 91 of 125 (or 73%) of the amino acids are identical in the Drosophila and chicken genes. These findings represent the first rigorous identification of a Drosophila proto-oncogene that can encode what may be a nuclear protein, and they set the stage for a genetic analysis of how c-myb serves the normal organism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens / genetics
  • Chromosome Mapping
  • Cloning, Molecular
  • Drosophila melanogaster / genetics*
  • Female
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Oncogenes*
  • Proteins / genetics
  • Saccharomyces cerevisiae / genetics
  • Transcription, Genetic
  • X Chromosome

Substances

  • Proteins

Associated data

  • GENBANK/M11281