USP13 promotes acute myeloid leukemia cell proliferation and autophagy by promoting ATG5

Yuchu Yuan; Meizhu Xue; Feng Zhou; Lifang Gu

doi:10.1016/j.tice.2024.102494

USP13 promotes acute myeloid leukemia cell proliferation and autophagy by promoting ATG5

Tissue Cell. 2024 Aug 9:91:102494. doi: 10.1016/j.tice.2024.102494. Online ahead of print.

Authors

Yuchu Yuan¹, Meizhu Xue², Feng Zhou³, Lifang Gu¹

Affiliations

¹ Inspection Division, Changshu blood bank, Changshu, Jiangsu 215500, China.
² Department of Paediatrics, Changshu NO.1 People's Hospital, Changshu, Jiangsu 215500, China. Electronic address: mz_xue0921@163.com.
³ Inspection Division, Changshu Medicine Examination Institute, Changshu, Jiangsu 215500, China.

PMID: 39216303
DOI: 10.1016/j.tice.2024.102494

Abstract

Objective: To elucidate the role of USP13 in acute myeloid leukemia (AML) by investigating its effects on cell growth, apoptosis and autophagy, and to explore the underlying mechanisms.

Methods: The expression of USP13 in AML cells was assessed using quantitative PCR (qPCR) and immunoblotting. Cell Counting Kit-8 (CCK-8) and Edu staining were employed to evaluate the impact of USP13 on AML cell growth. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunostaining assays were conducted to examine the effects of USP13 on apoptosis and autophagy in AML cells, and immunoblot assays were performed to determine the potential underlying mechanistic pathway.

Results: USP13 expression was significantly elevated in AML cells, correlating with enhanced cell proliferation and resistance to apoptosis. Moreover, USP13 promoted autophagy in AML cells. Mechanistically, USP13 was found to be associated with upregulating ATG5 expression, which promoted AML progression.

Conclusion: USP13 promotes AML cell growth and autophagy by upregulating ATG5.

Keywords: ATG5; Acute myeloid leukemia (AML); Apoptosis; Autophagy; USP13.