Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase

Nature. 1985 Apr 18-24;314(6012):592-7. doi: 10.1038/314592a0.

Abstract

The products of six unidentified reading frames of human mitochondrial DNA are precipitated from a mitochondrial lysate by antibodies against highly purified native beef heart NADH-ubiquinone oxidoreductase (complex I). These products are enriched greatly in a human submitochondrial fraction enriched in NADH-Q1 and NADH-K3Fe(CN)6 oxidoreductase activities. We conclude that the six reading frames encode components of the respiratory-chain NADH dehydrogenase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cytochrome Reductases / genetics*
  • DNA, Mitochondrial / genetics*
  • Genes
  • Humans
  • Intracellular Membranes
  • Macromolecular Substances
  • Molecular Weight
  • NAD(P)H Dehydrogenase (Quinone)
  • NADH Dehydrogenase / genetics*
  • NADH Dehydrogenase / immunology
  • NADH, NADPH Oxidoreductases / genetics*
  • Quinone Reductases / genetics*

Substances

  • DNA, Mitochondrial
  • Macromolecular Substances
  • NADH, NADPH Oxidoreductases
  • Cytochrome Reductases
  • NAD(P)H Dehydrogenase (Quinone)
  • Quinone Reductases
  • NADH Dehydrogenase