IL13RA2 promotes progression of infantile haemangioma by activating glycolysis and the Wnt/β-catenin signaling pathway

Oncol Res. 2024 Aug 23;32(9):1453-1465. doi: 10.32604/or.2024.048315. eCollection 2024.

Abstract

Background: Interleukin 13 receptor subunit alpha 2 (IL13RA2) plays an essential role in the progression of many cancers. However, the role of IL13RA2 in infantile haemangioma (IH) is still unknown.

Materials and methods: IL13RA2 expression in IH tissues was analyzed using western blot, qRT-PCR, and immunofluorescence. The role of IL13RA2 in haemangioma-derived endothelial cells (HemECs) was determined following knockdown or overexpression of IL13RA2 using CCK-8, colony formation, apoptosis, wound healing, tubule formation, Transwell, and western blot.

Results: IL13RA2 expression was upregulated in IH tissues. IL13RA2 overexpression promoted proliferation, migration, and invasion of HemECs and induced glycolysis, which was confirmed with a glycolysis inhibitor. Specifically, IL13RA2 interacted with β-catenin and activated the Wnt/β-catenin pathway in HemECs, which were involved in the above-mentioned effects of IL13RA2.

Conclusions: These findings revealed that targeting IL13RA2 is a potential therapeutic approach for IH.

Keywords: Glycolysis; IL13RA2; Infantile haemangioma; Wnt/β-catenin pathway.

MeSH terms

  • Cell Line
  • Cell Movement
  • Cell Proliferation
  • Glycolysis
  • Hemangioma* / genetics
  • Hemangioma* / metabolism
  • Hemangioma* / pathology
  • Humans
  • Infant
  • Interleukin-13 Receptor alpha2 Subunit* / genetics
  • Interleukin-13 Receptor alpha2 Subunit* / metabolism
  • Wnt Signaling Pathway*

Substances

  • IL13RA2 protein, human
  • Interleukin-13 Receptor alpha2 Subunit