Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain

Nature. 1985 May 23-29;315(6017):331-3. doi: 10.1038/315331a0.

Abstract

To date no nucleic acid has been found in the purified infectious agent which causes the spongiform encephalopathy known as scrapie. In an attempt to identify a unique scrapie virus-associated messenger RNA in tissues of infected animals, we have synthesized an oligonucleotide probe complementary to the mRNA sequence corresponding to the amino-acid sequence of the prion protein, PrP27-30 (ref. 1). We report here that, with this probe, a complementary DNA clone representing PrP27-30 was obtained from scrapie-infected mouse brain; the DNA sequence of this clone could be translated into a protein that matches exactly the published sequence of PrP27-30. The cDNA clone hybridized to a single 2.4-2.5-kilobase (kb) mRNA from both normal and scrapie-infected brain. Thus, the PrP27-30 mRNA is not uniquely associated with scrapie infectivity, suggesting that PrP27-30 may be a normal component of mouse and hamster brain.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / microbiology*
  • Brain / physiology
  • Cricetinae
  • Mice
  • Prions / genetics*
  • RNA, Messenger / genetics*
  • RNA, Messenger / isolation & purification
  • Viral Proteins / genetics*

Substances

  • Prions
  • RNA, Messenger
  • Viral Proteins