An immunocytochemical study on secretory mechanism of IgA in human pancreas

Acta Pathol Jpn. 1985 Jan;35(1):87-101. doi: 10.1111/j.1440-1827.1985.tb02208.x.

Abstract

To analyze the secretory mechanism of immunoglobulins into pancreatic secretion, pancreatic juice IgA was measured by single radial immunodiffusion and characterized immunochemically, and immunoglobulins, J chain and secretory component (SC) were localized immunocytochemically in pancreatic tissues by the peroxidase-labeled antibody method. The concentration of IgA was 0.0225 mg/ml with a range of 0.010 to 0.049. IgA associated with SC was demonstrated immunochemically in three cases. IgA, SC and J chain were demonstrated immunocytochemically in the pancreatic duct epithelium with the features characteristic of endocytic, SC-mediated transfer of IgA. The results of the present study suggested that transfer of IgA linked by J chain and SC across ductal epithelial cells, but not acinar cells occurred in human pancreas.

MeSH terms

  • Biological Transport
  • Endocytosis
  • Epithelium / metabolism
  • Epithelium / ultrastructure
  • Humans
  • Immunoglobulin A, Secretory / metabolism*
  • Immunoglobulin J-Chains / analysis
  • Immunoglobulins / analysis
  • Pancreas / immunology*
  • Pancreas / metabolism
  • Pancreatic Ducts / metabolism
  • Pancreatic Ducts / ultrastructure
  • Pancreatic Juice / immunology*
  • Secretory Component / analysis

Substances

  • Immunoglobulin A, Secretory
  • Immunoglobulin J-Chains
  • Immunoglobulins
  • Secretory Component