Il-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes in vivo

Cell. 1985 Jul;41(3):727-34. doi: 10.1016/s0092-8674(85)80053-2.


The continuously proliferating clones L/B AgA2, CB/Bm 7, Ba/C1, and Bc/Bm 11 were established from bone marrow of MRL/LPR, CBA/J, and BALB/c mice. These clones carry the B cell lineage surface antigen B-220 but not antigens normally expressed on mature B lymphocytes, myeloid cells, or T lymphocytes. Their immunoglobulin mu heavy chain and kappa light chain genes are in germ-line configuration. The G418 resistance gene was introduced into each clone with a retrovirus vector and then used as a selective marker for the progeny of transfected cells. Clones L/B AgA2, CB/Bm 7, and Bc/Bm 11, but not Ba/C1, could develop into antibody-secreting cells after in vivo transfer. None gave rise to cells responsive to polyclonal T cell activators, nor did any differentiate into cells that could develop into granulocyte/macrophage-colony-forming cells in vitro. All grew in interleukin 3 but not in other cytokines. We conclude that clones L/B AgA2, CB/Bm 7, and Bc/Bm 11 are early precursors of B lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Antigens, Surface*
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology*
  • Cell Differentiation
  • Cell Division
  • Clone Cells
  • Dexamethasone / pharmacology
  • Female
  • Genes
  • Gentamicins / pharmacology
  • Immunoglobulin Heavy Chains / genetics*
  • Immunoglobulin Light Chains / genetics*
  • Immunoglobulin kappa-Chains / genetics*
  • Immunoglobulin mu-Chains / genetics*
  • Interleukin-3
  • Lipopolysaccharides / pharmacology
  • Lymphocyte Activation
  • Lymphokines / pharmacology*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred CBA


  • Anti-Bacterial Agents
  • Antigens, Surface
  • Gentamicins
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Immunoglobulin kappa-Chains
  • Immunoglobulin mu-Chains
  • Interleukin-3
  • Lipopolysaccharides
  • Lymphokines
  • Dexamethasone
  • antibiotic G 418