NADPH-dependent generation of H2O2 in a thyroid particulate fraction requires Ca2+

FEBS Lett. 1985 Jul 1;186(1):107-10. doi: 10.1016/0014-5793(85)81349-1.


The action of Ca2+ on H2O2 formation catalyzed by an NADPH-dependent H2O2-generating system was studied in a thyroid particulate fraction. It was shown that H2O2 formation was inhibited by EGTA and restored in the presence of micromolar concentrations of free Ca2+, and that Mg2+ was unable to restore the activity of the EGTA-inhibited particulate fraction. Consequently, NADPH-dependent H2O2 formation requires Ca2+, and the Ca2+ stimulation of this process is specific and reversible.

MeSH terms

  • Animals
  • Calcium / pharmacology*
  • Egtazic Acid / pharmacology
  • Hydrogen Peroxide / metabolism*
  • Magnesium / pharmacology
  • NADP / pharmacology*
  • Swine
  • Temperature
  • Thyroid Gland / drug effects
  • Thyroid Gland / metabolism*
  • Thyrotropin / pharmacology


  • Egtazic Acid
  • NADP
  • Thyrotropin
  • Hydrogen Peroxide
  • Magnesium
  • Calcium