Molecular events during maturation of the immune response to oxazolone

Nature. 1985 Aug 1-7;316(6027):412-8. doi: 10.1038/316412a0.


Sequence analysis of the heavy- and light-chain messenger RNA of hybridomas immunized with a specific hapten yields important clues about the interplay between genetic and selective events during the onset and maturation of the immune response. The maturation of the primary response to the hapten 2-phenyl-5-oxazolone is characterized by a drift to higher-affinity somatic variants of a germline-encoded basic sequence, whereas hybridomas from the secondary response demonstrate a further maturation dominated by a shift to alternative germline combinations.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / genetics
  • Antibody Formation / drug effects*
  • Base Sequence
  • Hybridomas / drug effects
  • Hybridomas / immunology
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Light Chains / genetics
  • Mice
  • Oxazoles / pharmacology*
  • Oxazolone / pharmacology*
  • RNA, Messenger / genetics


  • Antibodies
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Oxazoles
  • RNA, Messenger
  • Oxazolone