Nucleotide sequence of the dihydrofolate reductase gene of methotrexate-resistant Lactobacillus casei

Gene. 1985;35(1-2):217-22. doi: 10.1016/0378-1119(85)90174-x.


The nucleotide sequence of the dihydrofolate reductase (DHFR) gene of a methotrexate-resistant strain of Lactobacillus casei, which is the source of DHFR for nuclear magnetic resonance (NMR) studies, has been determined. The derived amino acid sequence differs from that obtained by protein sequencing by the presence of aspartic acid instead of asparagine at position 8 and proline instead of leucine at position 90. The nucleotide sequences of 320-bp 5' and 335-bp 3' flanking regions of this gene have also been determined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Codon / genetics
  • DNA, Bacterial / genetics
  • Drug Resistance, Microbial
  • Genes, Bacterial*
  • Lactobacillus casei / drug effects
  • Lactobacillus casei / enzymology
  • Lactobacillus casei / genetics*
  • Methotrexate / pharmacology
  • Tetrahydrofolate Dehydrogenase / genetics*
  • Transcription, Genetic


  • Codon
  • DNA, Bacterial
  • Tetrahydrofolate Dehydrogenase
  • Methotrexate

Associated data

  • GENBANK/M10922