Alterations of lipid homeostasis in morbid obese patients are partly reversed by bariatric surgery

Flore Sinturel; Simona Chera; Marie-Claude Brulhart-Meynet; Jonathan Paz Montoya; Etienne Lefai; François R Jornayvaz; Giovanni D'Angelo; Minoa Karin Jung; Zoltan Pataky; Howard Riezman; Charna Dibner

doi:10.1016/j.isci.2024.110820

Alterations of lipid homeostasis in morbid obese patients are partly reversed by bariatric surgery

iScience. 2024 Aug 26;27(9):110820. doi: 10.1016/j.isci.2024.110820. eCollection 2024 Sep 20.

Authors

Flore Sinturel^{1

2

3

4}, Simona Chera⁵, Marie-Claude Brulhart-Meynet¹, Jonathan Paz Montoya^{6

7}, Etienne Lefai⁸, François R Jornayvaz^{2

3

9}, Giovanni D'Angelo⁷, Minoa Karin Jung¹⁰, Zoltan Pataky^{3

9}, Howard Riezman¹¹, Charna Dibner^{1

2

3

4}

Affiliations

¹ Division of Thoracic and Endocrine Surgery, Department of Surgery, University Hospitals of Geneva, 1211 Geneva, Switzerland.
² Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
³ Diabetes Center, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
⁴ Institute of Genetics and Genomics in Geneva (iGE3), 1211 Geneva, Switzerland.
⁵ Department of Clinical Science, University of Bergen, Bergen, Norway.
⁶ Proteomics Core Facility, EPFL, 1015 Lausanne, Switzerland.
⁷ Institute of Bioengineering, School of Life Sciences, EPFL, Lausanne, Switzerland.
⁸ INRA, Unité de Nutrition Humaine, Université Clermont Auvergne, Paris, France.
⁹ Division of Endocrinology, Diabetes, Nutrition, and Therapeutic Patient Education, Unit of therapeutic patient education, WHO Collaborating Centre, Department of Medicine, University Hospital of Geneva, 1211 Geneva, Switzerland.
¹⁰ Division of Visceral Surgery, Department of Surgery, University Hospital of Geneva, 1211 Geneva, Switzerland.
¹¹ Department of Biochemistry, Faculty of Science, NCCR Chemical Biology, University of Geneva, 1211 Geneva, Switzerland.

Abstract

Besides its beneficial effect on weight loss, gastric bypass surgery (GBS) may impact the circulating levels of phospho- and sphingolipids. However, long-term effects have not been explored. To investigate alterations in lipidomic signatures associated with massive weight loss following GBS, we conducted direct infusion tandem mass spectrometry on serum and subcutaneous adipose tissue (SAT) samples collected in a longitudinal cohort of morbid obese patients prior to GBS and 1 year following the surgery. A tissue-specific rearrangement of 13% among over 400 phospholipid and sphingolipid species quantified in serum and SAT was observed 1 year following GBS, with a substantial reduction of ceramide levels and increased amount of hexosylceramides detected in both tissues. The comparison of these new lipidomic profiles with the serum and SAT lipidomes established from an independent cohort of lean and morbid obese subjects revealed that GBS partly restored the lipid alterations associated with morbid obesity.

Keywords: Health sciences; Lipidomics; Medicine.