Screening of agents which convert 'transformed morphology' of Rous sarcoma virus-infected rat kidney cells to 'normal morphology': identification of an active agent as herbimycin and its inhibition of intracellular src kinase

Jpn J Cancer Res. 1985 Aug;76(8):672-5.

Abstract

During the course of screening of agents active in converting the transformed morphology of Rous sarcoma virus-infected rat kidney cells to the normal morphology, we identified an active substance produced by Streptomyces sp. MH237-CF8 as herbimycin. Herbimycin converted almost all cells into the normal morphology. The antibiotic was found to be an inhibitor of p60src-associated protein kinase in the cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Benzoquinones
  • Cell Transformation, Viral / drug effects*
  • Cells, Cultured
  • Kidney
  • Lactams, Macrocyclic
  • Oncogene Protein pp60(v-src)
  • Oncogene Proteins, Viral / antagonists & inhibitors*
  • Phenylglyoxal / analogs & derivatives
  • Protein-Tyrosine Kinases / antagonists & inhibitors*
  • Protein-Tyrosine Kinases / biosynthesis
  • Quinones / pharmacology*
  • Rats
  • Retroviridae Proteins / antagonists & inhibitors*
  • Retroviridae Proteins / biosynthesis
  • Rifabutin / analogs & derivatives

Substances

  • Benzoquinones
  • Lactams, Macrocyclic
  • Oncogene Proteins, Viral
  • Quinones
  • Retroviridae Proteins
  • Rifabutin
  • herbimycin
  • Protein-Tyrosine Kinases
  • Oncogene Protein pp60(v-src)
  • Phenylglyoxal
  • benzil