Bacterial cell wall composition, lysozyme resistance, and the induction of chronic arthritis in rats

Rheumatol Int. 1985;5(4):163-7. doi: 10.1007/BF00541517.


Bacterial cell wall induced arthritis is an experimental model of chronic erosive synovitis in which arthritis is induced in rats by a single injection of an aqueous suspension of cell wall fragments from selected Gram-positive bacteria. To understand better the Gram-positive bacterial cell wall characteristics necessary for the induction of chronic arthritis we tested the arthritogenicity of five Gram-positive bacteria which were (1) lysozyme resistant and contained a polyrhamnose peptidoglycan side chain moiety, (2) lysozyme resistant, but had little or no rhamnose in the peptidoglycan, polysaccharide, or (3) neither lysozyme resistant, nor contained rhamnose in their peptidoglycan, polysaccharide. All of the lysozyme resistant cell walls tested induced acute arthritis, but only those cell walls which were both lysozyme resistant and contained rhamnose in their polysaccharide side chain were able to induce chronic arthritis. Cell walls which were neither lysozyme resistant nor contained rhamnose were not arthritogenic. These data suggest that both lysozyme resistance and the rhamnose moiety in the peptidoglycan, polysaccharide side chain play an important role in the induction of chronic arthritis by Gram-positive bacterial cell walls in aqueous suspension.

MeSH terms

  • Animals
  • Arthritis / immunology*
  • Cell Wall / immunology
  • Disease Models, Animal
  • Female
  • Lactobacillus casei / immunology
  • Muramidase / metabolism
  • Polysaccharides, Bacterial / immunology
  • Polysaccharides, Bacterial / metabolism
  • Rats
  • Rats, Inbred Lew
  • Rhamnose / immunology
  • Structure-Activity Relationship


  • Polysaccharides, Bacterial
  • Muramidase
  • Rhamnose