Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes

EMBO J. 1985 Oct;4(10):2539-45. doi: 10.1002/j.1460-2075.1985.tb03968.x.

Abstract

The MRC OX-8 antigen is a marker of the rat cytotoxic T lymphocytes that consists of disulphide-linked chains of mol. wts. 37 and 32 kd. It is thought to be equivalent to the human T8 and mouse Lyt2,3 antigens (all now called CD8 antigens). MRC OX-8 antigen was purified from thymocytes using a monoclonal antibody column and because antigenicity was retained after reduction and alkylation the two polypeptide chains could be separated by a subsequent affinity chromatography step. Peptides were isolated from each chain and their sequences determined. A cDNA probe coding for the mouse CD8 antigen (pLY2C-1 provided by Dr L. A. Herzenberg) was used to obtain rat cDNA clones from which the sequence of the equivalent rat molecule was determined. Peptides from the 32-kd chain were identified in this translated sequence whereas peptides from the 37-kd chain were not. The 32-kd polypeptide sequence consisted of 210 amino acids and had one possible N-linked glycosylation site. The N-terminal part of the sequence was surprisingly different from both its mouse and human counterparts but, as in the other two species, it showed a clear relationship to Ig V domains.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface / genetics
  • Antigens, Surface / isolation & purification*
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Macromolecular Substances
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology
  • Membrane Proteins / isolation & purification*
  • Molecular Weight
  • Protein Sorting Signals
  • RNA, Messenger / genetics
  • Rats
  • T-Lymphocytes, Cytotoxic / analysis*

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • Macromolecular Substances
  • Membrane Proteins
  • Protein Sorting Signals
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/X03015