An alpha-like DNA polymerase from Halobacterium halobium

Eur J Biochem. 1985 Oct 15;152(2):293-7. doi: 10.1111/j.1432-1033.1985.tb09197.x.


Two DNA polymerases have been isolated from extracts of Halobacterium halobium, one having a sedimentation coefficient of 11 S, designated as alpha-like polymerase and possessing the following characteristics. It is sensitive to both aphidicolin and N-ethylmaleimide but indifferent to the presence of a dideoxynucleoside triphosphate. Therefore this polymerase is very similar to the alpha DNA polymerase of eukaryotes. The enzyme requires 5 M NaCl for maximum activity. The other polymerase has a sedimentation coefficient of 4.4 S and is resistant to both aphidicolin and N-ethylmaleimide. However, it is inhibited by a dideoxynucleoside triphosphate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aphidicolin
  • Centrifugation, Density Gradient
  • Chromatography, High Pressure Liquid
  • DNA Polymerase II / antagonists & inhibitors
  • DNA Polymerase II / isolation & purification*
  • Dideoxynucleotides
  • Diterpenes / pharmacology
  • Halobacterium / enzymology*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Sodium Chloride / pharmacology
  • Temperature
  • Templates, Genetic
  • Thymine Nucleotides / pharmacology


  • Dideoxynucleotides
  • Diterpenes
  • Thymine Nucleotides
  • Aphidicolin
  • Sodium Chloride
  • DNA Polymerase II
  • 2',3'-dideoxythymidine triphosphate