Stochastic processes drive the dynamic assembly of bacterial communities in Salix matsudana afforested soils

Front Microbiol. 2024 Sep 11:15:1467813. doi: 10.3389/fmicb.2024.1467813. eCollection 2024.

Abstract

Introduction: This study investigates the dynamic shifts in soil bacterial communities within a Salix matsudana afforested ecosystem transitioning from agricultural land. Understanding the temporal variability in bacterial diversity and community structures is crucial for informing forest management and conservation strategies, particularly in regions undergoing afforestation.

Methods: We employed high-throughput sequencing across three distinct months (August, September, and October) to analyze the temporal variability in bacterial community composition and diversity. Network analysis was utilized to identify keystone species and assess community stability under varying environmental conditions, including fluctuations in temperature and precipitation.

Results: We uncover significant temporal variability in bacterial diversity and community structures, which are closely tied to fluctuations in temperature and precipitation. Our findings reveal the abundance of the dominant bacterial phyla, such as Actinobacteria and Proteobacteria, which did not change overall, highlighting the stability and resilience of the microbial community across seasonal transitions. Notably, the increasing similarity in community composition from August to October indicates a reduction in species turnover, likely driven by more homogeneous environmental conditions. Through comprehensive network analysis, we identify the pivotal role of keystone species, particularly the human pathogen Nocardia, in maintaining community stability under reduced soil moisture. The observed variations in community connectivity underscore the microbial community's resilience and adaptability to seasonal shifts, with higher stability in August and October contrasting with the instability observed in September.

Discussion: These results underscore the complex interplay between stochastic and deterministic processes in bacterial community assembly, significantly shaped by prevailing environmental conditions. The insights gained from this research have far-reaching implications for forestry management and conservation strategies, particularly in regions undergoing similar afforestation efforts.

Keywords: community structure analysis; environmental influences; soil bacterial dynamics; soil pathogenic bacteria; temporal fluctuations.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was accomplished helped by the Hebei Science and Technology Innovation Base Project (246Z2903G), the Natural Science Foundation of Hebei Province (C2024205027), the Science Research Project of Hebei Education Department (QN2024266), the National Key Research and Development Program of China (2018YFC0506900), and the Science Foundation of Hebei Normal University (L2024ZD02 and L2023B26).