Ultrasound-assisted extraction, optimization, and purification of total flavonoids from Daphnegenkwa and analysis of their antioxidant, anti-inflammatory, and analgesic activities

Ultrason Sonochem. 2024 Dec:111:107079. doi: 10.1016/j.ultsonch.2024.107079. Epub 2024 Sep 23.

Abstract

Daphne genkwa (D. genkwa) is the dried flower buds of a Chinese medicinal plant with multiple biological activities. Response surface methodology (RSM) combined with artificial neural network (ANN) techniques were utilized to optimize ultrasound-assisted extraction conditions for D. genkwa. Antioxidant activity and anti-inflammatory and analgesic properties of total flavonoids from D. genkwa (TFDG) were assessed. Optimal conditions involving ultrasonic power of 225 W, 30 min extraction time, 30 mL/g liquid-solid ratio, 60 °C extraction temperature, and 70% ethanol concentration yielded a maximum total flavonoids content (TFC) of 5.41 mg/g. After microporous resin purification, four specific flavonoids in D. genkwa were identified and quantified using high-performance liquid chromatography (HPLC). The TFDG demonstrated potent antioxidant activity, with a 94% rate of scavenging the 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Furthermore, TFDG exhibited pain-alleviating properties in hot plate and acetic acid-induced writhing tests and noteworthy inhibitory effects on xylene-induced ear swelling in mice. The total flavonoids extracted by ultrasound had excellent biological activity. This establishes a foundation for further investigation into the potential medical value of D. genkwa.

Keywords: Artificial neural network; Daphne genkwa; Flavonoids; Response surface methodology; Ultrasonic.

MeSH terms

  • Analgesics* / chemistry
  • Analgesics* / isolation & purification
  • Analgesics* / pharmacology
  • Animals
  • Anti-Inflammatory Agents* / chemistry
  • Anti-Inflammatory Agents* / isolation & purification
  • Anti-Inflammatory Agents* / pharmacology
  • Antioxidants* / chemistry
  • Antioxidants* / isolation & purification
  • Antioxidants* / pharmacology
  • Chemical Fractionation / methods
  • Daphne* / chemistry
  • Flavonoids* / isolation & purification
  • Flavonoids* / pharmacology
  • Male
  • Mice
  • Pain / drug therapy
  • Ultrasonic Waves*

Substances

  • Flavonoids
  • Antioxidants
  • Analgesics
  • Anti-Inflammatory Agents