This study aimed to evaluate and compare the antioxidant capacity and enzyme inhibitory activity of extracts, sub-extracts, and fractions prepared from the aerial parts and roots of A. pseudocartalinica. The phytochemical content of the active extracts was also analyzed. According to the results, ellagic acid (38.42 mg/g) was the major compound in the aerial part methanol extract and catechin (185.30 mg/g) in the root methanol extract. The DPPH inhibition activity of all fractions was monitored, with the most active one (Fr B) reaching an IC50 value of (4.92±0.59 μg/mL). All the fractions prepared from the aerial parts' water sub-extract showed higher a-glucosidase inhibitory activity than the positive control acarbose. In the tyrosinase assay, Fr B (58.81±7.50 μg/mL) exhibited the highest inhibitory actions among all fractions. The structure of the major substances of the most active fraction were elucidated as quercetin 7-O-β-glucopyranosyl-3-O-β-glucuronopyranoside- (1), and α-[(2-formyl-5-hydroxymethyl)pyrrol-1-yl]aspartic acid (2).
Keywords: Alchemilla pseudocartalinica; Biological activity guided fractionation; Rosaceae; Tyrosinase; α-Glucosidase inhibitory.
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