Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma

Anal Biochem. 1985 Aug 15;149(1):166-8. doi: 10.1016/0003-2697(85)90490-7.


Rapid, large-scale isolation of human apolipoproteins A-I and A-II has been accomplished using two chromatographic procedures. The apolipoproteins adsorbed from plasma onto a column of phenyl-Sepharose are eluted with increasing propylene glycol concentrations. Apolipoproteins A-I and A-II can be resolved by elution with a linear 0 to 80% propylene glycol gradient. Homogeneous preparations of apo A-I and A-II are obtained following gel filtration in 3M guanidinium chloride.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Apolipoprotein A-I
  • Apolipoprotein A-II
  • Apolipoproteins A / blood*
  • Apolipoproteins A / isolation & purification
  • Chromatography, Agarose
  • Chromatography, Gel
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Sepharose / analogs & derivatives


  • Apolipoprotein A-I
  • Apolipoprotein A-II
  • Apolipoproteins A
  • phenyl-sepharose
  • Sepharose