Acinetobacter corruptisaponis sp. nov., Isolated from a Spoiled Bath Lotion

Abstract

Strain DM2021935^T representing a novel Acinetobacter species was isolated from a spoiled bath lotion in Guangdong, China. Based on 16S rRNA gene phylogenetic analysis, strain DM2021935^T was closely related to 'Acinetobacter thutiue' VNH17^T, Acinetobacter junii CIP 64.5 ^T, and Acinetobacter tibetensis Y-23 ^T. Cells of strain DM2021935^T were Gram-stain-negative, non-spore-forming, strictly aerobic, catalase-positive, oxidase-negative, α-hemolytic, and non-motile. Strain DM2021935^T exhibited growth in 1-3% (w/v) NaCl at temperatures ranging from 4 to 37 °C and tolerated pH levels from 6.0 to 8.0. The predominant fatty acids in strain DM2021935^T are C_12:0, C_16:0, C_18:1 ω9c, and summed feature 3. Polar lipid profiles included glycolipids, phospholipids, phosphatidylethanolamine, and phosphatidyl-N-methylethanolamine. The identified respiratory quinones were ubiquinone Q-8 and Q-9. The genomic size of DM2021935^T comprised 4.15 Mb, consisting of one chromosome (3,827,633 bp) and two plasmids (241,357 and 83,010 bp). The G + C content was 41.8%. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain DM2021935^T and phylogenetically related type strains were below the species delineation thresholds (72.2-95.4, 53.1-87.0, and 20.4-66.4%, respectively). AntiSMASH analysis identified four gene clusters: non-ribosomal peptide synthetase, non-alpha poly-amino group acids, YcaO cyclodehydratase, and aryl polyene biosynthesis. Based on genotypic data, strain DM2021935^T represents a novel species within the genus Acinetobacter. The proposed name for the novel species is Acinetobacter corruptisaponis sp. nov. (type strain DM2021935^T = KCTC 92772 ^T = GDMCC 1.3703 ^T).