Cloning of opal suppressor tRNA genes of a filamentous fungus reveals two tRNASerUGA genes with unexpected structural differences

EMBO J. 1985 Dec 16;4(13A):3553-6.

Abstract

The informational suppressors su4-1 and su8-1 of Podospora anserina were isolated by transformation of Schizosaccharomyces pombe UGA mutants. The DNA sequence revealed that they were opal (UGA) suppressor tRNAs. Wild-type alleles were also isolated by hybridization. The DNA sequence showed that they both encode species of tRNASerUGA. The gene SU8 has an 18-bp intervening sequence and its primary sequence is very different from that of SU4.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Fungi / genetics
  • Genes, Fungal*
  • RNA, Transfer / genetics*
  • Schizosaccharomyces / genetics
  • Sequence Homology, Nucleic Acid
  • Serine
  • Suppression, Genetic*

Substances

  • Serine
  • RNA, Transfer

Associated data

  • GENBANK/X05226
  • GENBANK/X05227