Extracellular vesicles are membrane-bound organelles that play crucial roles in intercellular communication and elicit responses in the recipient cell, such as defense responses against pathogens. In this study, we have optimized a protocol for isolating extracellular vesicles (EVs) from Sorghum bicolor apoplastic wash. We characterized the EVs using fluorescence microscopy and correlative light and electron microscopy. Key features • Allows the isolation of extracellular vesicles from the monocot plant Sorghum bicolor. • Labels isolated extracellular vesicles with fluorescent dyes for easy characterization with light microscopy. • Validates dye labeling and further characterizes extracellular vesicles using a correlative light and electron microscopy approach.
Keywords: Correlative light and electron microscopy; Extracellular vesicles; Monocot; Sorghum bicolor.
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