Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology

Tingyu Gong; Dandan Liu; Xiaochen Wang; Danni Zhou; Ling Tang; Hao Wang; Jun Su; Ping Liang

doi:10.1016/j.scr.2024.103580

Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology

Stem Cell Res. 2024 Dec:81:103580. doi: 10.1016/j.scr.2024.103580. Epub 2024 Oct 15.

Authors

Tingyu Gong¹, Dandan Liu², Xiaochen Wang², Danni Zhou², Ling Tang², Hao Wang³, Jun Su⁴, Ping Liang⁵

Affiliations

¹ Shulan International Medical College, Zhejiang Shuren University, Hangzhou, 310015, China.
² Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China.
³ Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China.
⁴ Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China. Electronic address: junsu@zju.edu.cn.
⁵ Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China. Electronic address: pingliang@zju.edu.cn.

PMID: 39426048
DOI: 10.1016/j.scr.2024.103580

Abstract

Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing technology was employed to establish a CIB1 knockout human embryonic stem cell line, which exhibited normal pluripotency and karyotype.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems*
Calcium-Binding Proteins* / genetics
Calcium-Binding Proteins* / metabolism
Cell Line
Gene Editing* / methods
Gene Knockout Techniques / methods
Human Embryonic Stem Cells / cytology
Human Embryonic Stem Cells / metabolism
Humans
Pluripotent Stem Cells / cytology
Pluripotent Stem Cells / metabolism

Substances

Calcium-Binding Proteins
CIB1 protein, human