The laser method for efficient introduction of foreign DNA into cultured cells

Exp Cell Res. 1986 Feb;162(2):372-8. doi: 10.1016/0014-4827(86)90342-3.

Abstract

A minute hole upon a cultured cell, perforated with a finely focused laser beam, was found to repair itself within a short period of time. The procedure constitutes a new way of introducing exogenous gene materials dissolved in medium into cells. The 'laser-aided' DNA transfection is better than the existing methods because it allows the treatment of a large number of cells in a shorter time, and an improved success rate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division
  • Cell Line
  • Cell Membrane Permeability
  • DNA*
  • DNA, Bacterial
  • Kidney
  • Lasers*
  • Rats
  • Transfection*
  • Transformation, Genetic*

Substances

  • DNA, Bacterial
  • DNA