Antiaging in a Bottle: Bioactive Competency of Plasma-Generated Nitric Oxide Water for Modulation of Aging-Related Signature in Human Dermal Cells

ACS Appl Mater Interfaces. 2024 Nov 6;16(44):59991-60007. doi: 10.1021/acsami.4c14051. Epub 2024 Oct 22.

Abstract

Nitric oxide (NO), a potential therapeutic antiaging molecule, modulates various physiological and cellular processes. However, alterations in endogenous NO levels brought on by aging impact multiple organ systems and heighten susceptibility to age-related skin diseases. This correlation underscores the importance of investigating NO-based antiaging interventions. Nonthermal plasma-generated NO is a promising avenue for cosmetic and regenerative medicine due to its capacity to stimulate cellular growth. Herein, we examine the potential of plasma-generated nitric oxide water (NOW) as a bioactive agent in human dermal fibroblasts, emphasizing gene expression patterns linked to extracellular matrix (ECM) breakdown and cellular senescence. The findings of our study indicate that administering NOW at lower dosages enhances cell migration and proliferation. Moreover, the genetic signatures associated with ECM synthesis, antioxidant defense, and antisenescence pathways have been analyzed in NOW-exposed cells. Notably, the downregulation of ECM-degrading enzyme transcripts─collagenase, elastase, and hyaluronidase─suggests NOW's potential in mitigating the intrinsic skin aging phenomena, emphasizing the promise of NO-based interventions in advancing antiaging strategies within regenerative medicine.

Keywords: ECM degradation; NOW; antiaging; antioxidants; collagen synthesis; nitric oxide.

MeSH terms

  • Cell Movement / drug effects
  • Cell Proliferation* / drug effects
  • Cellular Senescence* / drug effects
  • Extracellular Matrix* / drug effects
  • Extracellular Matrix* / metabolism
  • Fibroblasts* / drug effects
  • Fibroblasts* / metabolism
  • Humans
  • Nitric Oxide* / metabolism
  • Plasma Gases / chemistry
  • Plasma Gases / pharmacology
  • Skin / drug effects
  • Skin / metabolism
  • Skin Aging* / drug effects
  • Water* / chemistry

Substances

  • Nitric Oxide
  • Water
  • Plasma Gases