Characterization of DNA polymerase induced by salmon herpesvirus, Oncorhynchus masou virus

J Gen Virol. 1986 Feb;67 ( Pt 2):405-8. doi: 10.1099/0022-1317-67-2-405.

Abstract

A DNA polymerase induced by Oncorhynchus masou virus (OMV) was isolated from cultured salmon cells infected with OMV using sequential ion-exchange column chromatography steps. The properties of the OMV polymerase were compared to those of human cytomegalovirus (HCMV) polymerase and polymerase alpha from cherry salmon (masu salmon, O. masou) testes. OMV polymerase was clearly distinguished from the other polymerases by its optimum temperature for enzyme activity, 25 degrees C. In an investigation of the effects of phosphonoacetic acid and aphidicolin, OMV polymerase was found to be more resistant to both drugs than HCMV polymerase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cell Transformation, Viral
  • DNA-Directed DNA Polymerase / biosynthesis*
  • Female
  • Herpesviridae / enzymology*
  • Herpesviridae / genetics
  • Kinetics
  • Male
  • Ovary / enzymology
  • Salmon / microbiology*
  • Testis / enzymology
  • Thermodynamics

Substances

  • DNA-Directed DNA Polymerase