S-Adenosylmethionine Treatment Diminishes the Proliferation of Castration-Resistant Prostate Cancer Cells by Modulating the Expression of miRNAs

Arch Immunol Ther Exp (Warsz). 2024 Nov 1;72(1). doi: 10.2478/aite-2024-0022. eCollection 2024 Jan 1.

Abstract

AdoMet (S-adenosylmethionine) inhibits cancer cell proliferation and migration via epigenetic alterations. This study aimed to investigate whether AdoMet may cause alterations in microRNA (miRNA) expression profiles that are important for the initiation and progression of prostate cancer. PC-3 cells were treated with AdoMet before miRNA sequencing. A total of 17 differentially expressed miRNAs were detected. Target gene prediction was performed by means of databases. Results were aligned to transcriptomic data. The bioinformatic analysis revealed upregulation of anticancerogenic genes, downregulation of cancerogenic-related processes and pathways. Knocking down hsa-miR-192-5p in PC-3 cells resulted in downregulation of cancer cell proliferation, thus confirming these results.

Keywords: Ado-Met; PC-3 cells; TGF-beta pathway; miRNAs.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation* / drug effects
  • Computational Biology / methods
  • Epigenesis, Genetic
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic* / drug effects
  • Humans
  • Male
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • PC-3 Cells
  • Prostatic Neoplasms, Castration-Resistant* / drug therapy
  • Prostatic Neoplasms, Castration-Resistant* / genetics
  • Prostatic Neoplasms, Castration-Resistant* / metabolism
  • S-Adenosylmethionine* / metabolism

Substances

  • MicroRNAs
  • S-Adenosylmethionine