Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene

Gene. 1979 Dec;8(1):121-33. doi: 10.1016/0378-1119(79)90012-x.


We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2 mu circule. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Arginine / genetics
  • DNA, Fungal / genetics
  • Genes
  • Genetic Vectors*
  • Plasmids*
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic*


  • DNA, Fungal
  • Arginine