A chromosomal reference genome sequence for the malaria mosquito, Anopheles marshallii, Theobald, 1903

Boris K Makanga; Diego Ayala; Nil Rahola; Lemonde B A Bouafou; Harriet F Johnson; Haynes Heaton; Martin G Wagah; Joanna C Collins; Ksenia Krasheninnikova; Sarah E Pelan; Damon-Lee B Pointon; Ying Sims; James W Torrance; Alan Tracey; Marcela Uliano-Silva; Jonathan M D Wood; Katharina von Wyschetzki; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Shane A McCarthy; Daniel E Neafsey; Alex Makunin; Mara K N Lawniczak

doi:10.12688/wellcomeopenres.22989.1

A chromosomal reference genome sequence for the malaria mosquito, Anopheles marshallii, Theobald, 1903

Wellcome Open Res. 2024 Sep 26:9:554. doi: 10.12688/wellcomeopenres.22989.1. eCollection 2024.

Authors

Boris K Makanga¹, Diego Ayala^{2

3}, Nil Rahola^{2

4}, Lemonde B A Bouafou^{2

4}, Harriet F Johnson⁵, Haynes Heaton⁶, Martin G Wagah⁷, Joanna C Collins⁷, Ksenia Krasheninnikova⁷, Sarah E Pelan⁷, Damon-Lee B Pointon⁷, Ying Sims⁷, James W Torrance⁷, Alan Tracey⁷, Marcela Uliano-Silva⁷, Jonathan M D Wood⁷, Katharina von Wyschetzki⁷; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Shane A McCarthy^{7

8}, Daniel E Neafsey^{9

10}, Alex Makunin⁷, Mara K N Lawniczak⁷

Affiliations

¹ Département de Biologie et Écologie Animale, Institut de Recherche en Écologie Tropicale, Libreville, Gabon.
² MIVEGEC, University of Montpellier, CNRS, IRD, Montpellier, France.
³ Medical Entomology Unit, Institut Pasteur de Madagascar, Antananarivo, Antananarivo Province, Madagascar.
⁴ ESV, Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon.
⁵ Scientific Operations, Wellcome Sanger Institute, Hinxton, England, UK.
⁶ CSSE, Auburn University, Auburn, Alabama, USA.
⁷ Tree of Life, Wellcome Sanger Institute, Hinxton, England, UK.
⁸ Department of Genetics, University of Cambridge, Cambridge, England, UK.
⁹ Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
¹⁰ Infectious Disease and Microbiome Program, Broad Institute, Cambridge, Massachusetts, USA.

Abstract

We present a genome assembly from an individual female Anopheles marshallii (the malaria mosquito; Arthropoda; Insecta; Diptera; Culicidae) from Lopé, Gabon. The genome sequence is 225.7 megabases in span. Most of the assembly is scaffolded into three chromosomal pseudomolecules with the X sex chromosome assembled. The complete mitochondrial genome was also assembled and is 15.4 kilobases in length.

Keywords: African malaria mosquito; Anopheles marshallii; chromosomal; genome sequence.

Grants and funding

WT_/Wellcome Trust/United Kingdom