CRISPR-Cas9 Genome Editing in Auxotrophic and Non-auxotrophic Fission Yeast Strains

Aki Hayashi; Jun-Ichi Nakayama; Katsunori Tanaka

doi:10.1007/978-1-0716-4168-2_11

CRISPR-Cas9 Genome Editing in Auxotrophic and Non-auxotrophic Fission Yeast Strains

Methods Mol Biol. 2025:2862:155-170. doi: 10.1007/978-1-0716-4168-2_11.

Authors

Aki Hayashi¹, Jun-Ichi Nakayama^{2

3}, Katsunori Tanaka⁴

Affiliations

¹ Division of Chromatin Regulation, National Institute for Basic Biology, Okazaki, Japan. ahayashi@nibb.ac.jp.
² Division of Chromatin Regulation, National Institute for Basic Biology, Okazaki, Japan.
³ Basic Biology Program, Graduate Institute for Advanced Studies, Okazaki, Japan.
⁴ Department of Biosciences, School of Biological and Environmental Sciences, Kwansei Gakuin University, Sanda, Japan.

PMID: 39527199
DOI: 10.1007/978-1-0716-4168-2_11

Abstract

The CRISPR/Cas system is a very powerful genome-editing tool that has been developed over the past decade to optimize genome editing for many organisms. Here, we describe a rapid genome-editing method for fission yeast using the CRISPR-Cas9 system. It allows rapid generation of desired auxotrophic and non-auxotrophic strains without perturbing the local genome content by avoiding the insertion of selection markers at target loci.

Keywords: Auxotrophic; CRISPR-Cas9; Genome editing; Inducible promoter; Non-auxotrophic.

MeSH terms

CRISPR-Cas Systems*
Gene Editing* / methods
Genome, Fungal
RNA, Guide, CRISPR-Cas Systems / genetics
Schizosaccharomyces* / genetics

Substances

RNA, Guide, CRISPR-Cas Systems